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A new species of Keroplatidae (Diptera: Sciaroidea), Macrocera rohaceki sp. nov., is described from the island of Mallorca (Spain). A further remarkable species, Paleoplatyura johnsoni Johannsen, 1910, hitherto only known from the Nearctic Region, is recorded from Sicily (Italy). New records of Isoneuromyia czernyi (Strobl, 1909) and I. pseudochracea (Landrock, 1925) from Slovakia are provided, including fi gures of the male terminalia. Their relationships to the widely distributed European species, I. semirufa (Meigen, 1818), are reconstructed using three mitochondrial DNA markers (12S, COI, cytb). A key to the European species of Isoneuromyia Brunetti, 1912 is also provided.

Macrocera rohaceki sp. nov. and other interesting records of Keroplatidae (Diptera) from southern and central Europe, with DNA sequence data