English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Largely in apomictic tropical grasses molecular markers analyses including identifying genes through association genetics approach requires DNA from large numbers of samples in short span of time. A simple protocol for DNA isolation from 5 apomictic tropical grass species namely Dichanthium annulatum, Heteropogon concortus, Sehima nervosum, Chrysopogon fulvus and Cenchrus glaucus is described. The method allows DNA extraction from as little as 0.2 to 0.3 g of leaves ground in liquid nitrogen, followed by DNA isolation in 1.5 ml Eppendorf tubes involving modified cetyltrimethylammonium bromide (CTAB) procedure using 1% polyvinylpyrrolidone (PVP) to remove polysaccharides. By using the method, a DNA yield up to 345 μg/g leaf tissues was obtained. The quality of the DNA was quite suitable not only for PCR-based markers analyses but also for restriction enzyme digestion. No or insignificant DNA was obtained in plant samples extracted from the fixed solution (alcohol, alcohol and chloroform, alcohol and ethylene diamine tetra acetic acid (EDTA)), thus the use of liquid nitrogen was inevitable.

A Rapid Protocol for Isolating Genomic DNA from Tropical Grass Species Suitable for RAPD, ISSR and STS