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In the present investigation a method is demonstrated using bulk samples of genomic DNA to estimate the genetic relatedness among heterogeneous lucerne cultivars through RAPD markers. Each of 7 random primers employed generated 5 to 10 DNA fragments from the individual plant of 5 cultivars as well as bulked samples made of several plants per cultivar. The genetic distance (D) calculated from RAPD patterns obtained with bulked DNA samples ranged from 0.03 to 0.11 which largely corresponds to their known relatedness. The RAPD profiles of the bulk samples containing DNA from different numbers of individuals of the cultivars indicated bulking of 10 individuals DNA are necessary to get the representation of the most of the bands of the cultivars. Thus, the method demonstrated could be useful for estimating genetic relatedness among the heterogeneous crop like lucerne.

Bulk Genomic DNA PCR Analysis-A Rapid Method to Estimate Genetic Relatedness among Heterogeneous Lucerne (Medicago sativa L.) Cultivars