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Chlorophyllin, a sodium-copper salt derivative of chlorophyll a and b was evaluated at concentrations of 6.25, 12.5 and 25 μg/ml with regard to its clastogenic and anticlastogenic potential in Chinese hamster ovary cells CHO-k1 (wild-type) and CHO-xrs5 (DNA repair-defective), using a chromosomal aberration test. Cells were subjected to continuous exposure for 12 h to chlorophyllin in the presence or absence of ethyl methane sulfonate (EMS, 310 μg/ml). The results demonstrate that under the experimental conditions utilized, chlorophyllin was not cytotoxic nor clastogenic in either of the cell systems employed. Moreover, anticlastogenic activity was also not observed.

Investigation of Chlorophyllin in CHO-k1 (wild-type) and CHO-xrs5 (repair-deficient) Cells by a Chromosomal Aberration Assay