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A tetraploid variety of A. ampulacea was produced through colchiploidization by treatments in 0.15% and 0.20% colchicine for 7 to 20hr. The cytogenetics of the diploid and induced tetraploid were studied in order to determine the chromosome numbers and observe the meiotic behaviour. A. ampulacea diploid had a somatic chromosome complement of 18 chromosomes and regular 9 bivalents. The induced tetraploid had 36 somatic chromosomes and showed irregular meiosis characterized by the presence of quadrivalents and univalents at diakinesis and metaphase I with laggards and bridges at anaphse I. These differences in chromosomes numbers were reflected in plant size, length of spikes, number of spikelets and total seed production.

Colchiploid Induction in Anthephora ampulacea Stapf and Hubb.