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A cold shock treatment (16°C) on fertilized eggs of Marsilea vestita induces important ultrastructural alterations. The transfer of eggs from 16°C to 24°C allowed all organelles, plastids, mitochondria, endomembranes and ribosomes to recover a normal organization.However, the return to optimal conditions (24°C) induces the formation of nuclear bodies and the individualization of bundles of microfilaments near the nucleolus.Comparison of our obtained results and investigations concerning other materials indicates a great sensitivity to temperature of Marsilea eggs which rapidly react to weak variations.

Ultrastructural recovery of early embryos of Marsilea vestita (Pterophyta) after a cold shock treatment.