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The cellular slime mold Dictyostelium discoideum is an excellent organism for studying the mechanisms of cell differentiation and development. However, their sexual process is still obscure (Wilson 1952, 1953, Wilson and Ross 1957, Sussman 1961b, Olive 1963, Loomis and Ashworth 1968, Sinha and Ashworth 1969). Since the cellular slime mold has a unique life cycle, a few general points regarding it should be mentioned. D. discoideum is an unicellular organism which has amoeboid shape. The cells grow by feeding on bacteria and reproduce themselves by mitotic division. On starvation, many cells come together to aggregate, and form a slug-like shaped mass, called a pseudoplasmodium. The slug migrates a while, and forms a fruiting body which consists of spores and stalk cells. In general, it has been believed that they were normally haplonts through whole their life cycle.

Appearance of Wild Type from Mixed Culture of Two Aggregateless Mutants in the Cellular Slime Mold <i>Dictyostelium discodieum</i>