English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

In vitro fertilization and cultivation of mammalian ova has been singled out as one of the most challenging and frustration research projects in the field of reproductive biology. According to Austin (1961a, 1933), in spite of numerous attempts to fertilize mammalian ova in vitro, valid reports have appeared only sporadically. However, the cultivation of mammalian ova under conditions of various media have been reported to be successful since the pioneering works of Long (1912), Brachet (1913), Lewis and Gregory (1929), Pincus (1930), and Nicholas and Hall (1942). Most of the cultivated ova were started from two or more cell stages, and few attempts to cultivate fertilized ova following in vitro fertilization have been reported. In a previous experiment, Suzuki and Mastroianni (1965) have reported that rabbit ova can be fertilized in vitro in rabbit tubal fluid. A total of 337 ova were inseminated in vitro in rabbit tubal fluid under mineral oil using capacitated spermatozoa recovered from the uterus 12 hours after mating. The fresh tubal fluid was collected from ligated rabbit oviducts, diluted with Waymouth's medium and, for 124 of these, the mineral oil was pre. treated with 5% carbon dioxide in air. After four hours, ova were transferred to fresh tissue culture medium with added 10% rabbit serum and cultured for 18-20 hours. Ova were examined for evidence of sperm penetration, pronuclear formation, polar bodies, and cleavage. The fertilization rate was 30.9% without prior carbon dioxide equilibration and 63.7% when the diluted, carbon dioxide treated, oil was used. The purpose of the present investigation was to extend this previous work by cultivat ing these fertilized rabbit ova in vitro to more cell stages in suitable conditions.

<i>In Vitro</i> Cultivation of Rabbit Ova Following <i>In Vitro</i> Fertilization in Tubal Fluid