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Now it is a well established fact that the nucleolus is developed at certain definite loci of definite chromosomes (Heitz 1931 a, b, Gates 1937, Matsuura 1938, etc.). In this connection it will be interesting to study the nucleolus-chromosome relationship in Paris verticillatct MB.,1 since this plant comprises several distinct karyotypes. Descriptions of the four diploid and of the two triploid karyotypes were given in detail in a previous paper (Haga 1937). The fifth diploid karyotype (2n-V) is not reported before. This new karyotype is charac terized by the following features. One of the B-type chromosome pair is increased in its short arm length (B+), one of the C-type chromosome pair is deficient as to its short arm (C-), one of the D-type chromosome pair is deprived of its major portion of satellite (D-1) and in the other one satellite is entirely absent including its stalk (D-) (Fig. 1). Though the critical measurement has not been attempted, chro mosome C and D are apparently the same as those found in the other karyotypes pre viously described. B+ and D-1 are the new type chromosomes. All the karyotypes hither to known are shown with the chromosome symbols in the following tabulation, where 2n-I represents the standard diploid karyotype (cf. Figs. 1-2, and Haga 1937). Fig. 1. New diploid karyotype, 2n-V. A coo plete set of

Nucleolus-Chromosome Relationship in <i>Paris verticillata</i> MB., with Special Reference to the Presence and Absence of a Satellite