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The analysis of the specific activity of free, ester sterol and corticosterone in the subcellular components of rat adrenal gland after incubation with acetate-1-14C revealed that the mitochondrial free sterol seems to be the main source of the corticosterone biosynthesized in that tissue. However, the specific activity of corticosterone isolated from the incubated tissue was higher, irrespective of the incubation conditions used, than that of mitochondrial free sterol. This may suggest that a fraction of mitochondrial free sterol appeared to be drawn upon for corticosterone biosynthesis.

STUDIES ON THE BIOSYNTHESIS OF STEROL AND CORTICOSTERONE IN RAT ADRENAL GLAND