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As a part of physiochemical studies on muscular contraction, the authors have tried to follow up the distribution of antigenic protein into the muscular tissues of the guinea pig, as for anaphylactic contraction mechanism of uterus in guinea pig. To study the possible role of antigen-antibody reaction and estrogen or gestagen on the process, experiments were carried out on sensitized and non-sensitized animals with and without ovariectomy. As antigen parotin was used, since its biological properties as a physiologically active specific protein are well known. The I131 labelling method and the bodily distribution of I131-labelled parotin have already been studied (Tanaka and Ito, 1958; Tanaka et al., 1958 a and b) and it has also been demonstrated that I131-labelled serum-parotin presents the same immunological reaction as serum-parotin itself (Aonuma and Mimura, 1962). Therefore the authors used I131-labelled parotin for this immunological experiment.

endocrinologia japonica

THE DISTRIBUTION OF I&lt;SUP&gt;131&lt;/SUP&gt;-LABELLED PAROTIN INTO THE MUSCLE TISSUE OF NORMAL AND OVARIECTOMIZED GUINEA PIG SENSITIZED WITH PAROTIN