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1. Effects of thyroxine upon the succinic oxidase system of homogenates of heart muscle and adrenal cortex of the dog were pursued with wide range of concentration of the hormone.2. With the heart muscle homogenate, it was found that the range of thyroxine concentration, in which the enhancement of succinic oxidase activity was observed, was 0.1μg./ml. to 1μg./ml. Concentrations below 0.06μg./ml. were found ineffective, while those above 5μg./ml. up to 60μg./ml. were highly inhibitory.3. In the case of adrenal cortex, the concentration range in which the enhancement occurred, was found far above than that observed in heart muscle, i. e., between 4μg./ml. and 30μg./ml. 3μg./ml. caused no effect and 60μg./ml. inhibited the enzyme system.4. These range of thyroxine concentration were not substantially changed in the presence of high concentration of Na-and K-ions or in the absence of Ca-and Al-ions in the reaction mixture. Even when the washed homogenate (Green brei) of heart muscle was used, the effective range of thyroxine did not shift remarkably.

EFFECT OF THYROXINE UPON SUCCINIC OXIDASE SYSTEM OF HEART MUSCLE AND ADRENAL CORTEX OF DOG <I>IN VITRO</I>