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Complementary DNA (cDNA) encoding a long form of prolactin receptor (PRL-RL) was cloned from mouse mammary gland by PCR using primers designed from the noncoding regions of previously reported rat ovarian PRL-RL cDNA. The nucleotide sequence encoding the extracellular and transmembrane domains of PRL-RL is completely identical to that of short forms of mouse PRL-R. The amino acid sequence deduced from the nucleotide sequence of mouse PRL-RL is 91% identical to that of rat PRL-RL. To address the question of whether or not the cloned mouse PRL-RL cDNA encodes a functional PRL-RL we transfected Ba/F3 IL-3-dependent murine pro-B lymphoid cells with the cDNA. By culturing the transfected cells in a medium which contained PRL in place of IL-3, we selected 5 PRL-dependent clones. All of these PRL-dependent clones, BaF/PD cells, expressed PRL-RL mRNA. In addition, BaF/PD cells expressed mammary-specific gamma-casein mRNA in response to PRL and dexamethasone. Based on these results, it was concluded that the mouse mammary PRL-RL cDNA cloned in this study is functionally active in mediating both PRL-dependent growth and mammary-specific gene expression.

Prolactin-Dependent Growth and .GAMMA.-Casein Gene Expression in Ba/F3 Cells Transfected with a Long Form of Mouse Mammary Prolactin Receptor.