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The genetic, morophologic, and metabolic features of hereditary obese-hyperglycemic mice, strain C 57 BL/6 J-ob mice, have been well described by Dr . Jean Mayer et al. The present investigations were carried out in vitro for the purpose of extending the past finding. The results obtained are as follows : 1) Serum insulin-like activity of young OH mice increased markedly, but in older OH mice it decreased, more or less, lower than the level in their controls. 2) Glucose output by liver slice of OH mice was similar to those of their control mice when expressed per wet gram of liver. But the total liver function of adult OH mice was concluded to be approximately twice that of their control mice. 3) Epididymal adipose tissue from OH mice utilized less glucose than that of their control mice already in young age and the similar inclination was also found in older age. This was true in the absence of added insulin and in the presence of various doses of insulin. However, the sensitivity to insulin did not appear to be grossly disturbed. 4) Glucose uptake by diaphragm from young OH mice was at normal level, but decreased glucose uptake was found when old OH mice were used. The sensitivity to insulin was normal. 5) Release of NEFA from liver slice and diaphragm of young OH mice waf similar to those of their control mice, but decreased NEFA release was found when old OH mice were used. 6). Release of NEFA from epididymal adipose tissue of young OH mice was smaller than that obserbed in epididymal adipose tissue obtained from their control mice, but in fed status NEFA release from epididymal adipose tissue of OH mice was significantly

Studies on the Glucose and Lipid Metabolism and Their Related Hormones in the Hereditary Obese-hyperglycemic Mice