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Opuntia ficus-indica can be applied in bioproduct production because of its composition rich in carbohydrates. In this work, the liquid extract from stem structure (cladodes) were used for the selection of yeast with biotechnological interest. Candida utilis , C. lipolytica , Saccharomyces cerevisiae and Kluyveromyces marxianus were cultivated in this extract obtained after cactus treatment. Microbial growth aliquots were collected for analysis of biomass, sugars, pH and kinetic parameters. K. marxianus showed higher growth rate (μ= 0.25h -1 ) and higher percentage of consumption of sugar (95.68%). C. utilis obtained better biomass yield on the substrate consumed (Y x/s = 2.69). C. lipolytica was highlighted by showing a better kinetic profile, since it presented higher production of biomass (ΔX max = 10.93 g/l) and productivity within 24 hours of cultivation (P= 0.58 g/l.h -1 ) on the other yeasts tested. The influence of different salt concentrations in the growth of C. lipolytica in O. ficus-indica extract was tested with NaCl and KCl (0.1M, 0.25M, 0.5M, 1M, 2M and 4M). The growth under stress C. lipolytica was tolerant to saline concentrations (up to 1M NaCl and 0.5 M for KCl). The higher concentrations inhibited the cell growth. In the concentration of 4M was considered lethal to cells and sub-lethal 2M. Candida lipolytica cells might be able to adapt to osmotic stress by the concentration of 1M. The results suggest the use of this cactus specie as an alternative substrate for the microbial biomass production.

Utilização de leveduras para a produção de biomassa em extrato de palma forrageira (Opuntia ficus-indica)