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The choice of technology and bioinformatics' approach is central in the analysis of Next-Generation-Sequencing experiments. The pace with which new software and methodological guidelines are published, together with the fact that many of these choices will depend on the particularities of the study-system, mean researchers are often unable to produce informed decisions regarding these central questions. To address these issues, we introduce Pipeliner, a tool to simulate and validate the performance of NGS analysis pipelines, and optimize experimental designs.

Pipeliner: A tool to evaluate NGS pipelines and optimize experimental designs for resequencing studies.