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Materials and methods. Determination of acute toxicity LC50 of the test compound was performed in vivo on a model of the aquatic organism Zebrafish (Danio rerio) according to the instruction OECD No 203 (Fish, Acute Toxicity Test) for testing chemical compounds (acute toxicity test on fish from 10.12.2009). It was used fish 2 months of age, 11.8 ± 0.1 mm long, and weighing 2.6 ± 0.2 g in the experiment. The concentration of the test compound ranged from 5.0 mg/l to 100.0 mg/l. Test water-soluble compounds were dissolved in distilled water. Each mini-aquarium with a certain dose of the compound contained at least 7 individuals of Danio rerio. During the experiments, the fish were kept on a diet for a test 96 hours and their mortality was checked every 24, 48, 72 and 96 hours. Statistical analysis of the results was performed using the program Statistica 6.

A study of acute toxicity of newly synthesized compound on adult hydrobiont Danio rerio