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Materials and methods. Study of cytokine profile indexes was performed in 28 children with first diagnosed pulmonary tuberculosis from 1 to 16 years old (the average age was 9.2 ± 1.1 years). Depending on the specific process prevalence children with first diagnosed pulmonary tuberculosis were divided into two groups: the first group included 17 persons with a disseminated process and the second group included 11 persons with an isolated process. The comparison group included 30 healthy children. Comparison groups were age-matched and gender-matched. Cytokine profile indexes were studied by means of IL-2, IL-6, IL-4, IL-10 levels detection in blood serum through enzyme-linked immunosorbent assay using immunoenzymometric reader Sirio S and a set “Bender MedSystems GmbH” (Austria), (pg/ml). Dynamics of cytokine indexes was studied at the beginning of the antimycobacterial therapy intensive phase, on completion of the antimycobacterial therapy intensive phase (2 months after treatment) and on completion of the antimycobacterial therapy maintenance phase (6 months after treatment). Parents of all sick children signed patient’s written informed consent for participation in this study. Results of this work were processed by the modern methods of analysis with the help of a personal computer and the statistical package of the licensed software program Statistica® for Windows 6.0 (StatSoft Inc., No AXXR712 D833214FAN5).

Dynamics of cytokine profile indexes in children with first diagnosed pulmonary tuberculosis in the course of antimycobacterial therapy