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Chicken infectious anaemia virus (CIAV) is an economically important pathogen affect- ing poultry industry worldwide, and renders birds susceptible to secondary infections. The present study was designed to investigate the systemic immunosuppressive effects of CIAV on T lympho- cytes bearing CD4 and CD8 receptors using flow cytometry and hematological parameters during experimental subclinical infection in chicks. Forty specific pathogen free (SPF) chicks of 6 weeks of age were randomly and equally divided into two groups. Infected group received 10 4.5 TCID 50 of CIAV while control chicks were mock inoculated. All the chicks were regularly monitored for clinical signs, hematology parameters and CD4 + and CD8 + cell populations in spleen and blood. The experimental CIAV infection was confirmed by PCR testing of the tissue samples of experimental chicks, using VP2 gene specific primers, which yielded an expected amplicon size of 651 base pairs. The analysis of the hematological parameters showed significant decline in hematocrit value (PCV), total leukocyte count (TLC) and peripheral lymphocyte count (PLC) after 15 days post infection (dpi) but with no clinical signs of CIA. Flow cytometric analysis revealed that the percentage of CD4 + CD8 - and CD4 - CD8 + T cells significantly decreased in the virus infected chicks at 15 dpi both in the spleen and blood (p<0.05). The results supported the fact that subclinical CIAV infections are also immunodepressive in nature; the virus replicates in primary lymphoid tissues and induces immunosuppression by decreasing both CD4 + and CD8 + T cells in chicks.

advances in animal and veterinary sciences

Immunosuppressive Effects of Chicken Infectious Anaemia Virus on T Lymphocyte Populations Using Flow Cytometry and Hematological Parameters During Experimental Subclinical Infection in Chicks