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目的  观察A型肉毒素对家兔股二头肌的致萎缩作用。方法  以新西兰大耳白兔为实验对象分别以大(6.0U)、中3.0U、小(1.0U) 3种剂量的A型肉毒素注射于一侧股二头肌以另侧股二头肌注射同剂量生理盐水为对照观察其致萎作用的效果及持续时间并在显微镜下观察股二头肌的形态学变化。结果  家兔股二头肌局部注射A型肉毒素14d后可观察到明显的肌肉萎缩28d后肌肉萎缩稳定且以6.0U剂量组效果最佳。形态学观察显示单个肌纤维直径变小肌纤维融合边界不清晰排列不规则粗纤维/细纤维比值变小肌束间间隙增宽数量增加细胞核增多、聚集核与核之间距离缩小。结论  局部肌肉注射A型肉毒素致萎效果好模型稳定是一种较为理想的单个目的肌肌萎缩造模方法。

A型肉毒素对家兔股二头肌致萎缩作用的实验观察