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ABSTRAK Waduk Jatibarang terletak di Kecamatan Gunungpati, Kota Semarang. Waduk tersebut digunakan sebagai pengendali banjir, tempat pariwisata, kegiatan menangkap ikan, dan rencana akan dibuat sebagai sumber air bersih melalui PDAM. Pemanfaatan waduk untuk berbagai keperluan kemungkinan dapat menurunkan kualitas air dari waduk tersebut. Penelitian ini mengkaji kesuburan perairan Waduk Jatibarang berdasarkan distribusi dan kelimpahan bakteri heterotrofik. Bakteri heterotrofik berperan penting dalam sistem perairan sebagai perombak dan meremineralisasi bahan-bahan organik menjadi komponen anorganik sederhana. Tujuan dari penelitian ini adalah untuk mengetahui distribusi kelimpahan bakteri heterotrofik, kandungan bahan organik total, hubungan kelimpahan bakteri heterotrofik dengan bahan organik total, dan status kesuburan waduk Jatibarang. Metode yang digunakan dalam penelitian ini adalah metode deskriptif bersifat studi kasus.  Sampling dilakukan pada tanggal 27 April dan 11 Mei 2016 di lima (5) stasiun dengan kedalaman 1 meter. Penghitungan kelimpahan bakteri menggunakan metode Total Plate Count (TPC). Pengujian kandungan bahan organik total menggunakan metode Titrimetri Permanganat SNI 06-6989.22-2004. Hasil pengamatan menunjukkan distribusi spasial-temporal bakteri heterotrofik fluktuatif dan relatif tinggi, dengan kelimpahan berkisar (45 – 131) x 10 5 cfu/ml. Kandungan bahan organik total berkisar 11,771 - 21,883 mg/l. Hubungan antara bakteri heterotrofik dengan bahan organik total yaitu hubungan yang terbalik menunjukkan tidak adanya hubungan antara keduanya. Status kesuburan perairan Waduk Jatibarang berdasarkannilai kelimpahan bakteri heterotrofik (45 – 131) x 10 5 cfu/ml > (22 – 120) x 10 5 cfu/ml dan kandungan bahan organik total 11,771 – 21,883 mg/l perairan bersifat eutrofik. Kata kunci : Waduk Jatibarang; bakteri heterotrofik; bahan organik total; eutrofik ABSTRACT Jatibarang Reservoirs located in District Gunungpati, Semarang. The reservoir are used as flood control, the tourism, fishing activities, and the plan will be created as a source of clean water through the taps. Utilization of reservoirs for various purposes may reduce the quality of the water reservoir. This study examines the productivity waters Jatibarang Reservoir based on the distribution and abundance of heterotrophic bacteria. Heterotrophic bacteria play an important role in aquatic systems as to change and remineralize organic materials into simple inorganic components. The purpose of this study was to determine the distribution of the abundance of heterotrophic bacteria, total organic matter content, abundance of heterotrophic bacteria relationships with total organic matter and productivity status Jatibarang Reservoirs. The method used in this research is descriptive case study. The sampling was conducted on April 27 and May 11, 2016 in five (5) stations with a depth of 1 meter. Calculation of the abundance of bacteria using Total Plate Count (TPC). Tests using a total organic matter content titrimetric method SNI 06-6989.22-2004 permanganate. The results showed the spatial-temporal distribution of heterotrophic bacteria volatile and relatively high, with an abundance range (45-131) x 10 5 cfu/ml. Total organic matter content ranges (11.771 - 21.883) mg/l. The relationship between of heterotrophic bacteria with total organic material that is an inverse relationship showed no relationship between both. The productivity status of the dam water Jatibarang based on the abundance of heterotrophic bacteria (45-131) x 10 5 cfu/ml > (22-120) x 10 5 cfu/ml and organic matter content of the total 11.771 - 21.883 mg/l  waters are eutrophic. Keywords: Jatibarang Reservoir; heterotrophic bacteria; total organic matter; eutrophic

ANALISIS KESUBURAN PERAIRAN WADUK JATIBARANG BERDASARKAN DISTRIBUSI DAN KELIMPAHAN BAKTERI HETEROTROFIK