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Synthesis of Imine derivates used as antibacterial activity has been done. Synthesis was conducted by condensation reaction between piperonal and aniline. The product synthesis was characterized by melting point, LC-MS, FTIR and 1 H-NMR, then application of the product as antibacterial towards bacteria Staphylococcus aureus and Escherichia coli . The product of synthesis was pale-brown solid, and had melting point at 56-61 0 C. Identification by LC-MS showed the compound molecular weight was 225.30 gram/mol, identification by FTIR showed a compound contained imine C=N at 1620.21 cm -1 , C-H aromatic at 1581.63 cm -1 , C sp3 -H at 2962.37 cm -1 from CH 2 , and C-O at 1265.30 cm -1 , Identification by 1 H-NMR showed a compound had a singlet peak at δ=6 ppm that proton was from the CH 2 , multiplet peak at δ=6.9-7.7 ppm proton from two aromatics and singlet peak at δ=8.4 ppm from proton imine. The minimum inhibitory concentration of the product towards Staphylococcus aureus and Escherichia coli was 1 mg/mL with diameter inhibition zone 2 mm and 3 mm. From these data it could be concluded that the compound 3,4- methlenedioxybenzilyimine was formed and it had ability as an antibacterial agent.

Synthesis and Antibacterial Testing of Imina Derivative Compounds from Piperonal and Anilin