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The aim of the present study was to conduct univariate, bivariate and multivariate (principal component analysis, PCA) approaches in the screening of tannin-containing plants from various collection sites for their CH 4 mitigating properties. Plant samples were obtained from various collection sites in different countries, i.e. Indonesia (n = 27 species), Mongolia (n = 14), Switzerland (n = 16) and Germany (n = 3). The plants were incubated in vitro with buffered-rumen fluid at 39 o C for 24 h. Total gas production was recorded as an indicator of feed quality and emission of CH 4 was measured. Results showed that, based on bivariate screening, generally, plants possessed low CH 4 production had low quality or low total gas production except Rhus typhina, i.e. 43 ml/200 mg DM. The loading plot of PCA showed that all phenolic fractions were in the opposite direction with CH 4 and total gas production. Plants clustered together in reverse direction to that of CH 4 were Bergenia crassifolia root and leaf, Swietenia mahagoni, Clidemia hirta, Peltiphyllum peltatum, Acacia villosa and R. typhina. It was conluded that, for tannin-containing plants, screenings based on univariate, bivariate and multivariate approaches in relation to ruminal CH 4 emission led to similar results

SCREENING APPROACHES FOR METHANE MITIGATING POTENTIAL OF TANNIN-CONTAINING PLANTS UNDER IN VITRO RUMEN ENVIRONMENT