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The holothuroid spawning can be succesful depend on varoius factors such as salinity, temperature, primary productivity and other physiological adaptation toward environment influence s . The salinity fluctuation is one of the environment factors, which influence and can cause various osmotic pressure of coelomic fluid of Holothoroid and also gonad maturity stage. Holothuroid ( sea cucumber ), Paracaudina australis , is overexploited in Kenjeran waters , Surabaya , East Java . The study was aimed to reveal relationship between gonad maturity stage and level of osmotic work of sea cucumber P . australis. Fifty samples of P . australis were collected monthly.  Coelomic fluid samples of approximately 200–1000 µ l were obtained from all samples using disposable insulin syringes. T here were 37 holothuroids classified in the late maturity (stage 5), 74 holothuroid in the earlier maturity stage (stage 1). Gonad maturity stage 5 (late maturity) could be reached on December 2016. Contrasly, the gonad maturity stage 1 (earlier maturity) was found on August 2016. The analysi s of coelomic fluid showed that the average value of level of osmotic work ranged 70  to 91 mOsm/L H 2 O in the earlier gonad maturity stage. Contrasly, in the late maturity, the average value of level of osmotic work ranged 118 to 156 mOsm/L H 2 O . There is relationship between gonad maturation stage and level of osmotic work. From stage of gonad maturity 1 to 5, there were upward trend level of osmotic work in coelomic fluid of P . australis. Keyword s : g onad m aturity, o smotic , Paracaudina australis

Relationship Between Stage of Gonad Maturity and Level of Osmotic Work of Sea Cucumber, Paracaudina australis