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This study was carried out to investigate the optimum rate of substrate composition in bottle cultivation of Grifola frondosa and had three rates of substrate composition of 67:11:22(T1), 68:15:17(T2) and 74:14:12(T3) as mixing rate of weight of dried oak sawdust, dried corn husk and dried bean-curd refuse. The rate of primordia formation of T3 was 65.8% which was lowest among all treatments. Contraction rate of disease of T1 was 9.8% which was highest among all treatments. Harvesting rate of T2 was 70.5% which was highest among all treatments. Fruit body weights per bottle of T1 and T2 were 85.5 g, 83.3 g respectively and there was not significant difference between those. Yield per 10,000 bottles of T2 was 587 kg and was 7%, 28% higher than those of T1 and T3, respectively. As a result, the rate of substrate composition of 68:15:17(T2) as mixing rate of weight of dried oak sawdust, dried corn husk and dried bean-curd refuse was appeared as optimum rate of substrate composition in bottle cultivation of Grifola frondosa..

Cultural characteristics according to different rates of substrate composition in bottle cultivation of Grifola frondosa