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The in vitro effect of several temperatures (15, 20, 25, 30 and 35°C) was studied on growth rate, spore production and spore germination of two different isolates of the fungus Beauveria bassiana (Bals.) Vuil. In addition, a number of artificial solid media (PDA, PSA, maize meal, wheat meal and rice agar media) were tested to detect the medium most suitable for this fungus in regards of growth rate, spore production and spore germination. The experiment showed that the radial growth diameter of colonies increased linearly between days 3 and 14 postinoculation under the experimental temperatures. The optimum temperature was 25°C without significant differences between the two isolates. At this temperature, yield production was about (20×10) spore/ml, and spore germination was around 80%, whereas the fungus failed to grow at 35°C. PDA was the best medium in general for sporulating and germination of the fungus B. bassiana with yield production of (20-25 ×10) spore/ml, and germination of ≈ 85% without significant differences between the two isolates.

ssrg international journal of agriculture and environment science

Laboratory Evaluation of the Effect of Temperature and Several Media on the Radial Growth, Conidia Production and Germination of the Fungus Beauveria Bassiana (Bals.) Vuil