Open AccessCorrelations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency
Author(s) -
Mi-Ja Park,
Seong-joon Joh,
Kang-Seuk Choi,
AeRan Kim,
Min-Goo Seo,
JaeYoung Song,
Seon-Jong Yun
Publication year - 2016
Publication title -
korean journal of veterinary research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.136
H-Index - 7
eISSN - 2466-1392
pISSN - 2466-1384
DOI - 10.14405/kjvr.2016.56.3.189
Subject(s) - virology , potency , hemagglutination assay , hemagglutination , neutralization , infectious bronchitis virus , virus , microbiology and biotechnology , biology , in vitro , biochemistry , titer
The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen’s kappa coefficient between the VN titer of 2 log10 and HI titer of 5 log2 was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.
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