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Objective : the purpose of this study was to evaluate the efficacy of disinfection of type III dental stone by immersion in 1% sodium hypochlorite and 0.25% peracetic acid at different periods of time (1, 5 and 10 min). Material and Methods : s ilicon dies were previously infected with strains of Bacillus subtilis for 15 min. Then , type III gypsum stone (Herodent, Vigodent COLTENE SA, Rio de Janeiro, Brazil ) was inserted into the cavities to obtain contaminated specimens. A sterile silicone die was used to obtain uncontaminated specimens. The specimens were separated into positive and negative control groups, and further divided into the following groups: blocks immersed in sterile physiologic solution for 1, 5 or 10 min; blocks immersed in 1% sodium hypochlorite for 1, 5 or 10 min; and blocks immersed in 0.25% peracetic acid for 1, 5 or 10 min. All the groups were double-plated and incubated at 37 ˚C for 24 h. Results : t he results were expressed in colony forming units (CFU/ml) and the data were submitted to the Kruskal-Wallis test followed by Dunn’s test. The results showed that immersion in 1% sodium hypochlorite and 0.25% peracetic acid resulted in complete disinfection of the test specimens at all test periods (p < 0.01), whereas immersion in saline did not provide effective disinfection. Conclusion : i t can be concluded that both 1% sodium hypochlorite and 0.25% peracetic acid provided effective disinfection in dental stone specimens immersed in the solutions described above, at different periods of time. Keywords Disinfection; Peracetic acid; Sodium hypochlorite; Stone casts.

Microbiological evaluation of dental stone casts after immersion in sodium hypochlorite and peracetic acid