English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

The heat shock phenomenon has been c h a r a c t e r i z e d i n c u l t u r e d f i b r o b l a s t s of the rainbow t r o u t , Salmo g a i r d n e r i i . The response was e l i c i t e d by one of two methods: temperature e l e v a t i o n or sodium a r s e n i t e exposure. The s t r e s s s i t u a t i o n s r e s u l t e d i n the r a p i d expression of a set of novel polypeptides, the heat shock polypeptides (hsps), normally absent i n t r o u t c e l l s . At l e a s t s i x hsps have been i d e n t i f i e d and molecular weights assigned; these are r e f e r r e d to as hsp30, hsp32, hsp42, hsp62, hsp70, and hsp87. T r a n s l a t i o n a l c o n t r o l on p r e e x i s t i n g mRNAs was observed i n c e l l s under prolonged a r s e n i t e exposure. The heat shock response i s a r e v e r s i b l e process i n t r o u t c e l l s . Two cDNAs, THS70.7 and THS70.14, encoding p a r t i a l i n f o r m a t i o n f o r two d i s t i n c t species of t r o u t hsp70 were i s o l a t e d and c h a r a c t e r i z e d . These sequences are i d e n t i c a l at 73.3% of the n u c l e o t i d e p o s i t i o n s i n t h e i r regions of overlap, and t h e i r degree of sequence conservation at the polypeptide l e v e l i s 88.1%. The two derived t r o u t hsp70 polypeptide sequences show extensive homology w i t h amino a c i d sequences f o r hsp70 from Drosophila and yeast. Southern b l o t a n a l y s i s of t r o u t t e s t i s DNA re v e a l s a small number of bands h y b r i d i z i n g to the hsp70 genes i n t h i s s p e c i e s . The t r o u t hsp70 cDNA sequences c r o s s h y b r i d i z e w i t h r e s t r i c t i o n fragments i n genomic DNA from HeLa c e l l s , bovine l i v e r , nematodes, and Drosophila. Northern b l o t a n a l y s i s of RNA from arsenite-induced RTG-2 c e l l s (the t r o u t c e l l l i n e ) , using the t r o u t hsp70 cDNAs as probes, re v e a l s the presence of three hsp70 mRNA species. Both heat shock and sodium a r s e n i t e r e s u l t i n r a p i d synthesis of t r o u t hsp70 mRNA. S i m i l a r l y the r e p r e s s i o n of hsp70 mRNA i s very r a p i d , e s p e c i a l l y during recovery from a temperature s t r e s s . An a r t i f a c t of cDNA c l o n i n g was i d e n t i f i e d , i . e . an IS-element (named T31) was i s o l a t e d and c h a r a c t e r i z e d as o r g i n a t i n g from a t r o u t cDNA l i b r a r y . However, f u r t h e r a n a l y s i s proved T31 to be a p r o k a r y o t i c mobile element that had i n s e r t e d i t s e l f i n t o pBR322 during the pre p a r a t i o n of the cDNA l i b r a r y .

Studies on an inducible gene system : the heat shock response in trout cells