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By gaz chromatography-mass spectrometry (GC-MS) technique based metabolite profiling of the methanol extract derived from leaves of Prunella vulgaris L. (Lamiaceae) was carried out. P. vulgaris was grown in climatic chamber. For every metabolomics profile, we estimated 102 target substances, and 41 of them were identified. Metabolite spectrum on vegetation stage is different than other stage by absent of groups: amino acids, terpenes and lipids. Comparison of the results of statistical analysis based on the data array, including all detected metabolites (metabolite profiling) show, that metabolomes of blossom and fructification stage of plants match often and only a small number of metabolites P. vulgaris at the this stage make it specific. It was studied the spatial heterogeneity in the structure of the metabolic network. The results of it are showed that metabolomes variety of the leaves from one plants much more than local variety in the range of parts of one leaf. Spatial distributions of metabolites of heterogeneity in a leaf have been illustrated on the example of threonic acid.

METABOLOMIC PROFILE AT THE DIFFERENT STAGES OF PRUNELLA VULGARIS (LAMIACEAE) ONTOGENESIS AT GROWING IN THE CLIMATE CHAMBER