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Background and Aim:  The present serodiagnosis of brucellosis in livestock is based on the whole cell or smooth lipopolysaccharide of the  Brucella  organism in which specificity is hampered by the cross-reactivity, especially with the antibodies against  Yersinia enterocolitica  O:9 organism. The problem can be addressed by screening for better immunodominant antigens. Hence, the present study was undertaken to screen protein antigens of  Brucella abortus  for their diagnostic potential in cattle brucellosis.    Materials and Methods:  Protein antigens of  B. abortus  (n=10) non-reactive to antibodies against  Y. enterocolitica  O:9 were selected, expressed in  Escherichia coli , assessed the reactivity of expressed recombinant proteins by Western blot, standardized indirect-enzyme-linked immunosorbent assay (ELISA) for detecting  Brucella  antibodies in cattle serum, and comparative evaluation was done.    Results:  All the selected protein antigens were expressed and in the Western blot with  Brucella  antibodies positive cattle serum, six recombinant ( Brucella  protein 26 [BP26], Cu-Zn Superoxide dismutase [SodC],  B. abortus  I-1885, Serine protease, Bacterioferritin, and  Brucella  Lumazine Synthase [BLS]) proteins showed reaction whereas none of the proteins showed reactivity with  Brucella  negative cattle serum. ELISA has been done using known  Brucella  positive and negative cattle sera samples (n=113 each) in which the performance of recombinant proteins in diagnosing brucellosis was in the order of BP26 > BLS > SodC followed by rest of the proteins. BP26 based ELISA was found to be better with area under the curve as 0.953, and diagnostic sensitivity, diagnostic specificity, and Youden’s index of 90.27%, 95.58%, and 0.8584, respectively, with the excellent agreement (k=0.85).    Conclusion:  BP26 could be a potential diagnostic antigen among the immunodominant proteins of  B. abortus  in ruling out  Y. enterocolitica  O:9 infection while diagnosing brucellosis in cattle herds.

Comparative evaluation of the immunodominant proteins of Brucella abortus for the diagnosis of cattle brucellosis