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Background and Aim:   Mycoplasma  infection in small ruminants is a serious problem in sheep and goat herds around the world. It is responsible for high economic losses and decreased animal productivity. This study aimed to highlight the clinical, histopathological, minimum inhibitory concentration (MIC), and molecular characterization of  Mycoplasma  species in sheep and goats in Menoufiya Governorate, Egypt.    Materials and Methods:  A total of 234 samples were collected; 104 samples were collected from pneumonic lung tissues from the abattoir, in addition, 10 and 20 samples collected from apparently and diseased sheep, respectively, and 40 and 60 samples were collected from apparently and diseased goats, respectively, which were subjected to isolation onto pleuropneumonia-like organism medium. Polymerase chain reaction (PCR), histopathological examination, and determination of the MIC were also performed.    Results:  Of 104 samples of lung tissues showing pneumonic lesions, 56 (53.84%) were positive for  Mycoplasma  isolation. The positive isolation of  Mycoplasma  from 10 and 20 samples from apparently and diseased sheep was 30% and 40%, respectively as well as the positive isolation of  Mycoplasma  wa s  17% and 56.66% out of 40 and 60 apparently healthy and diseased field goat’s cases, respectively. All the diseased sheep and goats showed respiratory manifestations, including cough, bilateral nasal discharge, conjunctivitis, and systemic reaction. Evaluation of the MIC for  Mycoplasma   ovipneumoniae  revealed that lincospectin and tylosin were the most effective antibiotics at 2.5 mg/mL. Histopathological examination of affected lung tissue showed extensive hemorrhagic pneumonia with extensive alveolar hemorrhage. The PCR technique proved to be a rapid, specific, and sensitive method for the detection of  M. ovipneumoniae  and  Mycoplasma arginini  at 390 and 326 bp, respectively.    Conclusion:   M. ovipneumoniae  and  M. arginini  were the most prevalent species associated with respiratory infections in sheep and goats in the study area. Further studies are needed to investigate the role of these species in dissemination of the disease within herds of small ruminants.

Clinical, histopathological, and molecular characterization of Mycoplasma species in sheep and goats in Egypt