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Newcastle disease (ND) is a highly contagious and wide spread viral disease of the avian species causing severe economic losses in domestic poultry, especially chickens [1]. This disease is still one of the most important diseases in poultry production worldwide and remains a major constraint against the development of both industrial and village poultry production in Asia in spite of control measures including vaccination which has been applied since 1950s. Its global impact is enormous and remains a major barrier to international trade in poultry and poultry products and a greater drain on the world economy. The causative agent of the disease is Newcastle disease virus (NDV) also designated asAvian paramyxovirus serotype 1, which is a negativesense single-stranded RNA virus and belongs to the genus within the family [2]. The disease can vary from clinically in apparent to highly virulent forms, depending on the virus strain and the host species. NDV detection and pathotyping of avian isolates are extremely important because the appearance of virulent virus has significant economic consequences related to vaccination and eradication, impairing the ability of a given geographic region to export poultry products [3]. The widespread presence of lentogenic strains in feral birds and the use of such viruses as live vaccines mean that isolation of NDV is not enough to confirm a disease diagnosis and compliance with statutory requirements that may be in place [4]. Viral characterization using the pathogenicity test or nucleotide sequencing is also required, as the importance and impact of a given NDV isolate are directly related to its virulence. Hence analysis of a given field disease solely may be an unreliable measurement of pathogenicity due to the possibility of concurrent infections and bad technical management, laboratory assessments of the virus pathogenicity are necessary. Avulavirus Paramyxoviridae Veterinary World, EISSN: 2231-0916 Available at www.veterinaryworld.org/Vol.7/July-2014/3.pdf

Isolation and characterization of Newcastle disease virus from vaccinated commercial layer chicken