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Growing human population, increasing urbanization and rising per capital income are predicted to double the demand for the supply of livestock products in developing countries over the next two decades [1]. Livestock production is growing faster than any other sub-sector and it is predicted that by 2020, livestock will produce more than half of the total global agricultural output in terms of value [2]. The buffalo occupies an integral part in the livestock economy of Asia as well as of India and India has highest buffalo population in the world [3]. Buffaloes are major contributors for milk, meat and draught power and the buffalo is considered as the animal of the future in India due to their ability to survive inharshclimaticconditions, in comparison with cattle. However, reproductive efficiency of this species is low and hampered due to delayed puberty, silent estrus, prolonged inter calving intervals, poor conception rates, seasonal anoestrus and poor super ovulatory response [4]. Reproduction lies at the heart of any livestock breeding enterprise and is vital to maintain or increase the number of animals required for production. In order to improve reproductive efficiency of buffalo; assisted reproductive technologies such as artificial insemination (AI), multiple ovulation and embryo transfer (MOET) and production of embryos has been introduced [4]. Progress in the use of embryo transfer in the buffalo is slow due to poor super ovulatory response. embryo production (IVEP) would be an effective technique to improve the efficacy of transferable embryo production [5]. However, embryo production systems in buffalo are still sub optimal when compared with bovine and requires substantial improvement. in vitro

veterinary world/veterinary world

Effect of harvesting technique and presence or absence of corpus luteum on in vitro development after parthenogenetic activation of oocytes recovered from buffalo ovaries