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Plasmodiophora brassicae, the protozoan pathogen that causes clubroot on cabbage plants, is now a significant problem in oilseed rape crops. Due to numerous scientific studies in which the soil containing the spores of this pathogen is used, thorough sterilization of the infected medium has become very important. The aim of the study was to determine the possibility of effective sterilization of the soil and to define the factors affecting the viability of pathogen spores. The soil contaminated with P. brassicae spores, was subjected to the following processes: combustion, autoclaving, sterilization in the Koch apparatus, exposure to increased temperature, freezing at –80°C, disinfection with sodium hypochlorite and fungicides. The study showed that combustion, autoclaving and high temperature operation were the most effective ways to destroy spores in soil. Heating the soil showed that temperatures already above 70°C affected spore viability, while deep freezing of infected soil was completely ineffective. The use of Altima 500 SC significantly reduced plant infection.

Methods of soil sterilization contaminated with spores of Plasmodiophora brassicae, causal agent of clubroot