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Essential oils are complex, volatile compounds of plants origin, mainly obtained by steam distillation or extraction of the plant parts, i.e. seeds, leaves, fruits, bark or roots. These compounds exhibit a broad biological activity e.g. antimicrobial including fungistatic effect. The aim of the presented work was to determine the antifungal activity of 20 essential oils against chosen fungi: Alternaria alternata, Alternaria brassicicola, Botrytis cinerea and Sclerotinia sclerotiorum. The tests performed in 96-well microtiter plates were set up to define the minimum inhibitory concentration. Based on the obtained results it can be concluded that the tested essential oils exhibit antifungal activity towards examined filamentous fungi, however the MIC (Minimal Inhibitory Concentration) value was differentiated depending on the essential oil and fungal species. The lowest MIC values were obtained for lemon, grapefruit, cinnamon and oregano essential oils.

Fungistatic activity of essential oils towards selected oilseed rape pathogens