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Cercospora leaf spot (CLS) caused by Cercospora beticola is one of the most serious diseases of sugar beet (Beta vulgaris L.). C. beticola has been rapidly developing resistance to commercially used fungicides. In recent years strobilurin has been intensively used to control CLS and this induced an increase of C. beticola resistance to this group of fungicides. The aim of the study was to identify sensitive and resistant strains based on the RFLP analysis in plant material collected in different regions in Poland. Application of RFLP technique allowed for quick monitoring of pathogen population, eliminating fungus isolation and time-consuming tests on culture media. The results showed the presence of either sensitive or resistant strains in some regions, mixed strains in others. The obtained results confirmed rapid changes in population structure and increase in the frequency of G143A mutation.

The use of RFLP analysis in the monitoring of Cercospora beticola strobilurin resistance occurring in sugar beet (Beta vulgaris L.)