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Summary Brome mosaic virus (BMV) is spherical virus belonging to Bromovirus genera in Bromoviridae family. In Poland the virus was detected and described for the first time in wheat (Triticum aestivum L.) in 1997. In 2013 its presence was confirmed in triticale (× Triticosecale Wittm. ex A. Camus) and for the first time in maize (Zea mays L.). BMV is transmitted by vectors: mainly beetles (several species of Oulema and Phylotrella genera) and mechanically. The virus induce leaf mosaic of infected plants and reduce plant height and weight. The incidence of the disease is closely correlated with the beetles number. The existing literature data do not contain any information on the vectors infectivity in Poland. The specific primers and RT-PCR (reverse transcription-polymerase chain reaction) conditions were developed for the detection of BMV in its vector – cereal leaf beetle (Oulema melanopus L.). They can be used in the studies on the evaluation of caught beetles infectivity which will allow estimate the real risk of the disease.

Using RT-PCR assay for the detection of Brome mosaic virus (BMV) in cereal leaf beetle (Oulema melanopus L.)