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Isolation of nucleic acids of different plant pathogens is one of the most important stages, which have influence on the correct molecular diagnosis of these pathogens. This study investigated the suitability of two different DNA isolation techniques used in the diagnosis of Clavibacter michiganensis ssp. sepedonicus (Cms) – quarantine bacteria responsible for potato ring rot. The quality of the isolated DNA and the sensitivity of the two methods were assessed by spectrophotometric analysis, respectively/and the sensitivity of electrophoretic DNA PCR (polymerase chain reaction) assay. Two methods of DNA extraction were compared. The first of them with CTAB (cetyltrimethylammonium bromie) method allowed to remove the components of plant tissues and while destructing membranes released nucleic acids from bacterial cells (method 1). In the second method extraction of genomic DNA was done in the presence of high concentrations of salt, which involved the selective precipitation of nucleic acids (method 2). In addition to the efficiency of the compared extraction methods the impact of bacterial slime and buffer components on the quality of the obtained DNA were also assessed. It has been shown that the best quality of DNA and PCR sensitivity was obtained using the CTAB method. The high mucoid level of tested strains only marginally reduced the detection of bacteria Cms in PCR assay.

Influence of different methods of bacterial nucleic acid isolation on sensitivity of the PCR assay