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Summary High Resolution Melting Analysis (PCR-HRM – Polymerase Chain Reaction-High Resolution Melting) is a recently developed technique for fast, high-throughput post-PCR analysis of genetic mutations or variance in sequences of nucleic acid. It enables researchers to rapidly detect and categorize genetic mutations, identify new genetic variants without sequencing, or determine the genetic variation in a population prior to sequencing. PCR-HRM technique was also used to detect and distinguish Bursaphelenchus xylophilus from morphologically and genetically similar nematode species B. mucronatus . In the research reported here PCR-HRM was conducted on the rDNA of B. xylophilus (China) and Polish isolates of B. mucronatus (Mdz-01, Rad-03 and Wro-01), with the use of primers specifically designed from the ITS-1 region of B. mucronatus . The conducted study revealed that PCR-HRM is an effective essay which allows one to distinguish of B. xylophilus from B. mucronatus . This technique is very sensitive and enables detection of differences even in single nucleotides. PCR-HRM technique is also much cheaper when compared to commonly used PCR-RFLP.

The use of PCR-HRM technique for detection of the quarantine nematode Bursaphelenchus xylophilus