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fig 1. c-rerexpresslon In transverse (A, B, E) and coronal (C, 0, F) CNS sections. (A) E13.5 mouse embryo. Spinal motor neurons (mns) and dorsal root ganglia j()"g) expressc-l8l1ransatpts. (B) Mesencephalic region of a neonate. Expression is detected In the vemral tegmental area (ViA) and the OOJIofooIt>'nucleus (III). ~q Caudal brain stem region 01a neona1e. Expression Is observed in the dorsal lTIOtor nucleus 01the vagus (dmnx),hypoglossal nucleus (XU), nucleus arnblguos (pm), spII1B.ltrigeminal nudeus (Soli); .retbJlar format'.on (rf) and A1JC1 8dreoer~ ceil groop. (0) Pontine region 01 a neonate. The locus C8fuleus (Ie), ladal (IIII), abducens (Vi)an~ mesencephalic tngttmlnal (MMV) nuclei express c-lBt. (E a.;d F) E12.5 and neonatal midbrain region respectlvety. c-rot staining IS observed in the VTAand substantia nigra (SN). alerent column, including the neural C~f..lt~erived mesencephaF:: trigeminaJ nucleus and the spinal trigeminal nucleus. c.,et transcripts were also observed in the reticular formajon arid the main monoaminergic systems of the brain stem: serotonergic Md catecholaminergic (dopaminergic, noradrenergic and adrenergic). Among these systems, the highest levels of c-ret expression were detected ir.

The effect of the ret- mutation on the normal development of the central and parasympathetic nervous systems.