English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The purpose of this study is to develop an apparatus for simultaneous measurement of electrical and spectroscopic parameters of single ion-channels. We have combined the single channel recording apparatus with an artificial lipid bilayer and a fluorescence microscope designed to detect single fluorescent molecules. The artificial membranes were formed on a PEG (polyethylene glycol)-coated glass and observed with an objective-type total internal reflection fluorescence (TIRF) microscope. The lateral motion of a single lipid molecule (β-BODIPY 530/550 HPC) was recorded. The lateral diffusion constant of the lipid molecule was calculated from the trajectories of single molecules as D=8.0± 4.0× 10-8 cm2/s. Ionic channels were incorporated into the membrane and single-channel current fluctuations were recorded. These data show the possibility of the present technique for simultaneous measurement of electrical and spectroscopic parameters of single-channel activities. [DOI: 10.1380/ejssnt.2005.70]

An artificial lipid bilayer formed on a PEG-coated glass for simultaneous electrical and optical measurement of single ion-channels