Open AccessDirect Saliva Transcriptome Analysis
Author(s) -
Yu-Hsiang Lee,
Hui Zhou,
Jean Reiss,
Xinmin Yan,
Lei Zhang,
David Chia,
David T. Wong
Publication year - 2011
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2010.159210
Subject(s) - saliva , transcriptome , glyceraldehyde 3 phosphate dehydrogenase , gene expression , biology , microbiology and biotechnology , gene , biochemistry
Current standard operating procedures for salivary transcriptomic analysis require low temperatures and lengthy mRNA isolation, which substantially hamper its use in the clinic. We developed a streamlined, ambient-temperature processing, stabilization, and storage protocol for clinical analysis of salivary RNA.
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