Open AccessMultiplex Enzyme Assay for Galactosemia Using Ultraperformance Liquid Chromatography–Tandem Mass Spectrometry
Author(s) -
DaeHyun Ko,
Sun-Hee Jun,
HyungDoo Park,
Sang Hoon Song,
Kyoung Un Park,
Jin Q Kim,
YoungHan Song,
Junghan Song
Publication year - 2010
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2009.139618
Subject(s) - galactosemia , chemistry , chromatography , tandem mass spectrometry , galactose , enzyme , multiplex , high performance liquid chromatography , mass spectrometry , enzyme assay , liquid chromatography–mass spectrometry , biochemistry , biology , bioinformatics
Galactosemia is one of the most important inherited disorders detected by newborn screening tests. Abnormal results in screening tests should be confirmed by enzyme activity assays, but existing methods are time and labor intensive. We developed a novel multiplex enzyme assay for galactosemia using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
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