Open AccessDNA Sequence Capture and Enrichment by Microarray Followed by Next-Generation Sequencing for Targeted Resequencing: Neurofibromatosis Type 1 Gene as a Model
Author(s) -
Lan-Szu Chou,
C-S Jonathan Liu,
Benjamin Boese,
Xinmin Zhang,
Rong Mao
Publication year - 2009
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2009.132639
Subject(s) - biology , reference genome , pseudogene , computational biology , dna sequencing , genetics , dna microarray , genome , gene , gene expression
The introduction and use of next-generation sequencing (NGS) techniques have taken genomic research into a new era; however, implementing such powerful techniques in diagnostics laboratories for applications such as resequencing of targeted disease genes requires attention to technical issues, including sequencing template enrichment, management of massive data, and high interference by homologous sequences.
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