Open AccessQuantitative Transcription Factor Analysis of Undifferentiated Single Human Embryonic Stem Cells
Author(s) -
Anders Ståhlberg,
Martin Bengtsson,
Martin Hemberg,
Henrik Semb
Publication year - 2009
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2009.131433
Subject(s) - homeobox protein nanog , sox2 , biology , embryonic stem cell , transcription factor , homeobox , microbiology and biotechnology , gene expression profiling , single cell analysis , gene expression , cell , induced pluripotent stem cell , genetics , gene
Human embryonic stem cells (hESCs) require expression of transcription factor genes POU5F1 (POU class 5 homeobox 1), NANOG (Nanog homeobox), and SOX2 [SRY (sex determining region Y)-box 2] to maintain their capacity for self-renewal and pluripotency. Because of the heterogeneous nature of cell populations, it is desirable to study the gene regulation in single cells. Large and potentially important fluctuations in a few cells cannot be detected at the population scale with microarrays or sequencing technologies. We used single-cell gene expression profiling to study cell heterogeneity in hESCs.
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