Open AccessMeasurement and Quality Control Issues in Multiplex Protein Assays: A Case Study
Author(s) -
Allison A. Ellington,
Iftikhar J. Kullo,
Kent R. Bailey,
George G. Klee
Publication year - 2009
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2008.120717
Subject(s) - multiplex , analyte , univariate , control chart , computer science , sample (material) , data mining , bioinformatics , chemistry , multivariate statistics , chromatography , biology , machine learning , process (computing) , operating system
Multiplex arrays are increasingly used for measuring protein biomarkers. Advantages of this approach include specimen conservation, limited sample handling, and decreased time and cost, but the challenges of optimizing assay format for each protein, selecting common dilution factors, and establishing robust quality control algorithms are substantial. Here, we use measurements of 15 protein biomarkers from a large study to illustrate processing, analytic, and quality control issues with multiplexed immunoassays.
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