Open AccessA Convenient LC-MS Method for Assessment of Glucose Kinetics In Vivo with d-[13C6]Glucose as a Tracer
Author(s) -
Haoyue Zhang,
Robert Stevens,
Sarah P. Young,
Richard S. Surwit,
Anastasia Georgiades,
Raymond C. Boston,
David S. Millington
Publication year - 2009
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2008.113654
Subject(s) - tracer , chemistry , in vivo , chromatography , isotope , electrospray ionization , isotopomers , carbohydrate metabolism , mass spectrometry , insulin , medicine , biochemistry , biology , microbiology and biotechnology , physics , organic chemistry , quantum mechanics , nuclear physics , molecule
The isotope-labeled intravenous glucose tolerance test (IVGTT) combined with computer modeling is widely used to derive parameters related to glucose metabolism in vivo. Most of these methods involve use of either (2)H(2)-labeled or (13)C(1)-labeled D-glucose as a tracer with GC-MS to measure the isotope enrichment. These methods are challenging, both technologically and economically. We have developed a novel approach that is suitable for labeled-IVGTT studies involving a large cohort of individuals.
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