Open AccessSnapback Primer Genotyping with Saturating DNA Dye and Melting Analysis
Author(s) -
Luming Zhou,
Roscoe Errigo,
Hongzhe Lu,
Mark A. Poritz,
Michael Seipp,
Carl T. Wittwer
Publication year - 2008
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2008.107615
Subject(s) - amplicon , primer (cosmetics) , genotyping , primer extension , microbiology and biotechnology , melting temperature , polymerase chain reaction , oligonucleotide , dna , chemistry , biology , genetics , genotype , materials science , base sequence , gene , composite material , organic chemistry
DNA hairpins have been used in molecular analysis of PCR products as self-probing amplicons. Either physical separation or fluorescent oligonucleotides with covalent modifications were previously necessary.
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