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Campylobacter  species are common foodborne pathogens associated with cases of human gastroenteritis worldwide. A detailed understanding of the prevalence, contamination levels and molecular characteristics of  Campylobacter spp . in cattle and chicken, which are likely the most important sources of human contamination, is imperative. A collection of 1243 poultry meat samples (665 chicken breasts and 578 chicken thighs) and 1203 bovine meat samples (689 hamburgers and 514 knife-cut meat preparations) were collected at retail outlets, in randomly selected supermarkets located in different Italian regions during one year. Of these samples, 17.38% of the poultry meat and 0.58% of the bovine meat samples tested positive for  Campylobacter , of which 131 were  Campylobacter jejuni  (57.96%) and 95 were  Campylobacter coli  (42.03%).  Campylobacter  isolates were genotyped with the aim of assessing the genetic diversity, population structure, source distribution and  Campylobacter  transmission route to humans. All isolates were molecularly characterized by pulse field gel electrophoresis (PFGE), and further genotyped using multilocus sequence typing (MLST) and fla-SVR sequencing to gain better insight into the population structure. Antibiotic resistance was also investigate. The highest levels of resistance among chicken strains were observed for ciprofloxacin (88.25%), nalidixic acid (81.45%) and tetracycline (75.6%). PFGE analysis revealed 73 pulsotypes for  C .  jejuni  and 54 pulsotypes for  C .  coli , demonstrating the existance of different and specific clones circulating in Italy. MLST of  C . jejuni  isolates mainly clustered in the CC353, CC354, CC21, CC206 and CC443; while C.coli isolates clustered only in CC828. The most common flaA alleles were 287 for  C .  jejuni  and 66 for  C .  coli . Our study confirms that poultry meat is the main source of  Campylobacter iosis, whereas red meat had a low level of contamination suggesting a minor role in transmission. The high presence of  Campylobacter  in retail chicken meat, paired with its increased resistance to antimicrobials with several multidrug resistance profiles detected, is alarming and represents a persistent threat to public health.

Thermotolerant Campylobacter spp. in chicken and bovine meat in Italy: Prevalence, level of contamination and molecular characterization of isolates